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rabbit anti mouse adiponectin igg  (Assaypro)


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    Assaypro rabbit anti mouse adiponectin igg
    Rabbit Anti Mouse Adiponectin Igg, supplied by Assaypro, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+adiponectin+igg/Mouse+Immunoglobulin+G+(IgG)+Antibody/pm18441469-4-34-38
    Average 91 stars, based on 1 article reviews
    rabbit anti mouse adiponectin igg - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Effects of metabolic risk factors on production of plasminogen activator inhibitor-1 and adiponectin by adipocytes.
    Article Snippet: The 3T3-L1 mouse pre-adipocytes were grown to confluence and allowed to differentiate into adipocytes in the presence of dexamethasone (0.25μmol/L), isobutylxanthine (0.5 mmol/L) and insulin (0.2μmol/L) (all sourced from Sigma Chemical Co, St Louis, MO, USA), as described by Lundgren et al.5 Then, the differentiated adipocytes were incubated for 24 h in fresh medium containing glucose (5.5 mmol/L, 16.5 mmol/L) with or without insulin (10nmol/L, 50nmol/L) and triglycerides (1% Intralipid®; Pharmacia & Upjohn, Somerset, NJ, USA). .. At the end of the cultured period, the PAI-1 and adiponectin concentrations were measured in the cultured medium by western blotting analysis, using specific antibodies: rabbit antimouse PAI-1 IgG (Molecular Innovations, Southfield, MI, USA); rabbit anti-mouse adiponectin IgG (ASSAYPRO, St Charles, MO, USA); and goat anti-rabbit Ig (Biosource Int, Camarillo, CA, USA). ..

    Western Blot:

    Article Title: Effects of metabolic risk factors on production of plasminogen activator inhibitor-1 and adiponectin by adipocytes.
    Article Snippet: The 3T3-L1 mouse pre-adipocytes were grown to confluence and allowed to differentiate into adipocytes in the presence of dexamethasone (0.25μmol/L), isobutylxanthine (0.5 mmol/L) and insulin (0.2μmol/L) (all sourced from Sigma Chemical Co, St Louis, MO, USA), as described by Lundgren et al.5 Then, the differentiated adipocytes were incubated for 24 h in fresh medium containing glucose (5.5 mmol/L, 16.5 mmol/L) with or without insulin (10nmol/L, 50nmol/L) and triglycerides (1% Intralipid®; Pharmacia & Upjohn, Somerset, NJ, USA). .. At the end of the cultured period, the PAI-1 and adiponectin concentrations were measured in the cultured medium by western blotting analysis, using specific antibodies: rabbit antimouse PAI-1 IgG (Molecular Innovations, Southfield, MI, USA); rabbit anti-mouse adiponectin IgG (ASSAYPRO, St Charles, MO, USA); and goat anti-rabbit Ig (Biosource Int, Camarillo, CA, USA). ..



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    (a) <t>Adiponectin</t> mRNA expression determined by quantitative real-time PCR in porcine corpora lutea, granulosa cells, and theca interna cells harvested on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. The results are expressed as means ± S.E.M. ( n = 5). Bars with different letters at the top are different ( P < 0.05). (b) Adiponectin protein expression determined by Western blotting analysis in porcine corpora lutea, granulosa, and theca interna cells on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. Upper panel: representative immunoblots (MM—molecular marker, G—granulosa cells, Th—theca interna cells); lower panel: densitometric analysis of adiponectin protein relative to actin protein. The noted values are expressed as means ± S.E.M. in arbitrary optical density units ( n = 5). Bars with different letters at the top are different ( P < 0.01).
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    (a) <t>Adiponectin</t> mRNA expression determined by quantitative real-time PCR in porcine corpora lutea, granulosa cells, and theca interna cells harvested on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. The results are expressed as means ± S.E.M. ( n = 5). Bars with different letters at the top are different ( P < 0.05). (b) Adiponectin protein expression determined by Western blotting analysis in porcine corpora lutea, granulosa, and theca interna cells on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. Upper panel: representative immunoblots (MM—molecular marker, G—granulosa cells, Th—theca interna cells); lower panel: densitometric analysis of adiponectin protein relative to actin protein. The noted values are expressed as means ± S.E.M. in arbitrary optical density units ( n = 5). Bars with different letters at the top are different ( P < 0.01).
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    FIG. 6. Circulating levels of SAA, insulin, leptin, and <t>adiponectin.</t> Plasma was collected from mice at 12 weeks after diet. A: SAA levels were measured in 12–15 mice from each group using enzyme-linked immunosorbent assay. B: Insulin levels were measured in 9–14 mice from each group using RIA. C: Leptin levels were measured in 9–11 mice from each group using RIA. D: Adiponectin levels were measured in six mice from each group by SDS-PAGE as described in RESEARCH DESIGN AND METHODS. The high–molecular weight (HMW) form of the protein was quantified using densitometric analysis and is expressed relative to the a/a;LDLR/ chow diet levels. Data are presented as means SE. Genotypes and statistics are indicated below graphs.
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    Image Search Results


    (a) Adiponectin mRNA expression determined by quantitative real-time PCR in porcine corpora lutea, granulosa cells, and theca interna cells harvested on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. The results are expressed as means ± S.E.M. ( n = 5). Bars with different letters at the top are different ( P < 0.05). (b) Adiponectin protein expression determined by Western blotting analysis in porcine corpora lutea, granulosa, and theca interna cells on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. Upper panel: representative immunoblots (MM—molecular marker, G—granulosa cells, Th—theca interna cells); lower panel: densitometric analysis of adiponectin protein relative to actin protein. The noted values are expressed as means ± S.E.M. in arbitrary optical density units ( n = 5). Bars with different letters at the top are different ( P < 0.01).

    Journal: International Journal of Endocrinology

    Article Title: Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

    doi: 10.1155/2014/957076

    Figure Lengend Snippet: (a) Adiponectin mRNA expression determined by quantitative real-time PCR in porcine corpora lutea, granulosa cells, and theca interna cells harvested on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. The results are expressed as means ± S.E.M. ( n = 5). Bars with different letters at the top are different ( P < 0.05). (b) Adiponectin protein expression determined by Western blotting analysis in porcine corpora lutea, granulosa, and theca interna cells on days 2-3, 10–12, 14–16, and 17–19 of the oestrous cycle. Upper panel: representative immunoblots (MM—molecular marker, G—granulosa cells, Th—theca interna cells); lower panel: densitometric analysis of adiponectin protein relative to actin protein. The noted values are expressed as means ± S.E.M. in arbitrary optical density units ( n = 5). Bars with different letters at the top are different ( P < 0.01).

    Article Snippet: To identify immunoreactive bands, membranes were incubated for 1.5 h at room temperature with mouse anti-rabbit IgG for adiponectin (Sigma, USA; diluted 1 : 2000), goat anti-rabbit IgG for actin (diluted 1 : 5000) conjugated with alkaline phosphatase (Santa Cruz Biotechnology, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Marker

    The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on basal progesterone secretion by cultured luteal cells collected on days 10–12 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Result are means ± S.E.M. of five replicates.

    Journal: International Journal of Endocrinology

    Article Title: Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

    doi: 10.1155/2014/957076

    Figure Lengend Snippet: The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on basal progesterone secretion by cultured luteal cells collected on days 10–12 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Result are means ± S.E.M. of five replicates.

    Article Snippet: To identify immunoreactive bands, membranes were incubated for 1.5 h at room temperature with mouse anti-rabbit IgG for adiponectin (Sigma, USA; diluted 1 : 2000), goat anti-rabbit IgG for actin (diluted 1 : 5000) conjugated with alkaline phosphatase (Santa Cruz Biotechnology, USA).

    Techniques: Cell Culture

    The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on insulin-stimulated (I; 10 ng/mL) and/or FSH-stimulated (10 ng/mL) progesterone secretion by cultured granulosa cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Journal: International Journal of Endocrinology

    Article Title: Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

    doi: 10.1155/2014/957076

    Figure Lengend Snippet: The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on insulin-stimulated (I; 10 ng/mL) and/or FSH-stimulated (10 ng/mL) progesterone secretion by cultured granulosa cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Article Snippet: To identify immunoreactive bands, membranes were incubated for 1.5 h at room temperature with mouse anti-rabbit IgG for adiponectin (Sigma, USA; diluted 1 : 2000), goat anti-rabbit IgG for actin (diluted 1 : 5000) conjugated with alkaline phosphatase (Santa Cruz Biotechnology, USA).

    Techniques: Cell Culture

    The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on basal oestradiol secretion by cultured granulosa cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Journal: International Journal of Endocrinology

    Article Title: Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

    doi: 10.1155/2014/957076

    Figure Lengend Snippet: The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on basal oestradiol secretion by cultured granulosa cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Article Snippet: To identify immunoreactive bands, membranes were incubated for 1.5 h at room temperature with mouse anti-rabbit IgG for adiponectin (Sigma, USA; diluted 1 : 2000), goat anti-rabbit IgG for actin (diluted 1 : 5000) conjugated with alkaline phosphatase (Santa Cruz Biotechnology, USA).

    Techniques: Cell Culture

    The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on basal testosterone secretion by cultured theca interna cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Journal: International Journal of Endocrinology

    Article Title: Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

    doi: 10.1155/2014/957076

    Figure Lengend Snippet: The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on basal testosterone secretion by cultured theca interna cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Article Snippet: To identify immunoreactive bands, membranes were incubated for 1.5 h at room temperature with mouse anti-rabbit IgG for adiponectin (Sigma, USA; diluted 1 : 2000), goat anti-rabbit IgG for actin (diluted 1 : 5000) conjugated with alkaline phosphatase (Santa Cruz Biotechnology, USA).

    Techniques: Cell Culture

    The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on insulin-stimulated (I; 10 ng/mL) and/or LH-stimulated (10 ng/mL) (a) progesterone, (b) oestradiol, and (c) androstenedione secretion by cultured theca interna cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Journal: International Journal of Endocrinology

    Article Title: Adiponectin Expression in the Porcine Ovary during the Oestrous Cycle and Its Effect on Ovarian Steroidogenesis

    doi: 10.1155/2014/957076

    Figure Lengend Snippet: The effect of adiponectin (10 μ g/mL-A10 and 1 μ g/mL-A1) on insulin-stimulated (I; 10 ng/mL) and/or LH-stimulated (10 ng/mL) (a) progesterone, (b) oestradiol, and (c) androstenedione secretion by cultured theca interna cells collected on days 17–19 of the oestrous cycle. Bars with different superscripts are significantly different ( P < 0.05). Results are means ± S.E.M. of five replicates.

    Article Snippet: To identify immunoreactive bands, membranes were incubated for 1.5 h at room temperature with mouse anti-rabbit IgG for adiponectin (Sigma, USA; diluted 1 : 2000), goat anti-rabbit IgG for actin (diluted 1 : 5000) conjugated with alkaline phosphatase (Santa Cruz Biotechnology, USA).

    Techniques: Cell Culture

    FIG. 6. Circulating levels of SAA, insulin, leptin, and adiponectin. Plasma was collected from mice at 12 weeks after diet. A: SAA levels were measured in 12–15 mice from each group using enzyme-linked immunosorbent assay. B: Insulin levels were measured in 9–14 mice from each group using RIA. C: Leptin levels were measured in 9–11 mice from each group using RIA. D: Adiponectin levels were measured in six mice from each group by SDS-PAGE as described in RESEARCH DESIGN AND METHODS. The high–molecular weight (HMW) form of the protein was quantified using densitometric analysis and is expressed relative to the a/a;LDLR/ chow diet levels. Data are presented as means SE. Genotypes and statistics are indicated below graphs.

    Journal: Diabetes

    Article Title: Diet-induced increases in adiposity, but not plasma lipids, promote macrophage infiltration into white adipose tissue.

    doi: 10.2337/db06-1375

    Figure Lengend Snippet: FIG. 6. Circulating levels of SAA, insulin, leptin, and adiponectin. Plasma was collected from mice at 12 weeks after diet. A: SAA levels were measured in 12–15 mice from each group using enzyme-linked immunosorbent assay. B: Insulin levels were measured in 9–14 mice from each group using RIA. C: Leptin levels were measured in 9–11 mice from each group using RIA. D: Adiponectin levels were measured in six mice from each group by SDS-PAGE as described in RESEARCH DESIGN AND METHODS. The high–molecular weight (HMW) form of the protein was quantified using densitometric analysis and is expressed relative to the a/a;LDLR/ chow diet levels. Data are presented as means SE. Genotypes and statistics are indicated below graphs.

    Article Snippet: Protein was transferred to nitrocellulose membranes and probed with rabbit anti-mouse adiponectin (ABCAM, Cambridge, MA) at a 1:2,000 dilution.

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, SDS Page, High Molecular Weight